recJ [2020-09-09 14:39:58]

single-strand DNA-specific exonuclease, acts together with [protein|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|RecQ] in replication fork maintenance

locus: BSU_27620

pI: 5.18

mw: 87.83 kDa

protein length: 786 aa

gene length: 2361 bp

function: replication fork maintenance

product: single-strand DNA-specific exonuclease

essential: no

synonyms: yrvE

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]

Gene

Coordinates: 2,823,419 → 2,825,779

Phenotypes of a mutant

• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
• suppression of lethality of [gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] inactvation [pubmed|32793628]

The protein

Protein family

• RecJ family (with [protein|CDFA18E1C0445302ED4D8F93F88822EC6BD646BB|YorK], according to UniProt)

Paralogous protein(s)

• [protein|CDFA18E1C0445302ED4D8F93F88822EC6BD646BB|YorK]

[SW|Domains]

• [SW|DHH-DHHA1 domain]

[SW|Cofactors]

• Mn²⁺ [pubmed|27058167]

Structure

• [PDB|5F54] (from Deinococcus radiodurans, the [SW|DHH-DHHA1 domain], 32% identity) [pubmed|27058167]

[SW|Localization]

• associated with the [SW|replisome] [pubmed|21170359]
• bound to replication forks, but also assembles at many sites on the nucleoid upon DNA damage induction [pubmed|30401797]

Expression and Regulation

Operons

• genes: [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]-[gene|38DF74800FC5D54183C58B0EB82BD9A3EB93EE33|apt]
  description: [Pubmed|15241682]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

additional information

• [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• MGNA-B516 (yrvE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1515 NBRP B. subtilis, Japan]
• GP895 (''[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]''::''kan''), available in [SW|Jörg Stülke]'s lab
• BKE27620 (''[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]
• BKE27620 (Δ[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA
• BKK27620 (Δ[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA

References

Reviews

• 22933559,32286623

Original publications

• 15241682,11948165,10498723,21170359,21205011,26001966,27058167,30401797,32793628